Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet: Serum levels of total and HDM-specific immunoglobulins show a time-of-day response and sex-based differences to chronic HDM exposure Total IgE, Total IgG, HDM-specific IgE, HDM-specific IgG, HDM-specific IgG1, HDM-specific IgG2b, HDM-specific IgM, and HDM-specific IgA in the serum of chronic PBS- and HDM-exposed females and males at ZT0 and ZT12 were determined by ELISA. Data were expressed as ng/ml and mg/ml for total IgE and total IgG, respectively. HDM-specific immunoglobulins (IgE, IgG, IgG1, IgG2b, IgM, and IgA) in the serum were determined by commercially available ELISA kits (Chondrex, Inc.). Unable to detect HDM-specific IgG2a and IgG3 levels in PBS- and HDM-exposed mice. Data for the HDM-specific immunoglobulins were expressed as absorbance at 450 nm or μg/ml. Data are shown as mean ± SEM, Two-way ANOVA followed by Tukey’s multiple comparison test (n = 5/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, compared to respective control (PBS) at ZT0 or ZT12; # p < 0.05, compared to HDM at ZT0 vs. ZT12; # # p < 0.01, compared to HDM at ZT0 vs. ZT12. Summary statistics for interaction between Treatment x Sex x Time were analyzed using generalized linear modeling using R (see Table S2 ).

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet:

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Purification, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer

doi: 10.1186/s13046-020-01564-4

Figure Lengend Snippet: PRLR-DbsAb stimulates T cell infiltration and the PD-L1 expression unregulated in tumor tissue. sc Tumor cells plus sc effector cells (E/T 1:4) model: ( a ) HE staining of tumor tissue strapped from mice treated with 3 mg/kg PRLR-DbsAb. The above image showed normal tumor tissue, and the following image showed degenerate necrotic tumor tissue; ( b ) Immunohistochemical staining of CD8 in tumor tissue from the mice of different group; ( c ) Immunohistochemical staining of PD-L1 in tumor tissue from the mice of different group. Sc tumor cells plus ip effector cells (1:1) model: ( d ) HE staining; ( e ) Immunofluorescent staining of CD8. Three independent experiments were conducted and representative data is shown in this figure

Article Snippet: Breast cancer cell lines (MDA-MB-231, MCF-7, SKBR-3, and T47D) were incubated with control isotype IgG, PE-conjugated mouse anti-Human PRLR (Sino Biological) or APC-conjugated mouse anti-Human PD-L1 (Sino Biological) on ice for 30 min and then washed twice with FACS buffer (PBS with 2% FBS).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer

doi: 10.1186/s13046-020-01564-4

Figure Lengend Snippet: PD-1 inhibition increases PRLR-DbsAb mediated cytotoxicity of PRLR expression target cell. FACS histograms of PDL1 expression on different breast cancer cell lines including MDA-MB-231 ( a ) and T47D ( e ). The gray histogram is the fluorescent signal of non-specific IgG control antibody. Freshly isolated PBMCs and the target cells of MDA-MB-231 ( b ) or T47D ( f ) were incubated with or without addition of PD-1 mAb in different concentrations of PRLR-DbsAb at the ratio of 10:1 (E: T) for 20 hours. MDA-MB-231 ( c ) and T47D ( g ) cells were treated with 100 ng/ml PRLR-DbsAb at different time points (3, 10 and 20h) with or without adding PD-1 mAb and LDH release was detected. The expression of PD-L1 ( d and h ) on the corresponding effector cells and target cells is measured by Flow cytometer, respectively. ( i ) FACS histogram of the PD-L1 expression of target T47D with or without addition of 100 ng/ml PRLR mAb. ( j ) PD-L1 expression on CD4+ and CD8+ T cells. PBMCs and target T47D cells were incubated with 100 ng/ml PRLR-DbsAb at the ratio of 10:1 (E: T) for 20 hours. Three independent experiments were performed and the data was represented as the Mean ±SEM. *, ** and *** respectively indicate a statistically significant difference of at least P <0.05, <0.01 and <0.001

Article Snippet: Breast cancer cell lines (MDA-MB-231, MCF-7, SKBR-3, and T47D) were incubated with control isotype IgG, PE-conjugated mouse anti-Human PRLR (Sino Biological) or APC-conjugated mouse anti-Human PD-L1 (Sino Biological) on ice for 30 min and then washed twice with FACS buffer (PBS with 2% FBS).

Techniques: Inhibition, Expressing, Isolation, Incubation, Flow Cytometry